Bacteriocin 3xBiotics Presentation

The antibacterial research, from the past 50 years, has focused on meeting the medical needs caused by life-threatening infectious pathogens. The indiscriminate use of synthetic antibiotics has led to the development of many germs that are resistant to them. Also a major problem with the administration of antibiotics in allopathic medicine, either externally or internally, is the creation of dysbiosis which leads to uncontrolled multiplication of pathogens (eg the development of oral/ vaginal candidiasis as a result of antibiotics).

Modification of the intestinal microbiota can lead to many physical and mental illnesses. In addition, synthetic antibiotics have many side effects as well as contraindications.

The advantage of using fermented medicinal plants is that they have a double effect on the pathogen and immunity, increased bioavailability, pre/ pro and postbiotic intake.

That’s why Pro Natura comes to the aid of patients with a new product – Bacteriocin 3xBiotics.

Bacteriocins1 are small peptides produced by beneficial bacteria (especially LAB), whose property is to destroy other bacteria by acting on them by blocking protein synthesis. Due to their mechanism of action, they represent a potential solution in solving the current crisis (increasing antibiotic resistance2).

Artemisia (Wormwood) is a medicinal plant, widely used in traditional medicine (from vaginal lavage, gastrointestinal disorders, cancers3), which produces secondary metabolites, has direct antimicrobial effects on many resistant pathogenic bacteria, such as: Mycobacterium tuberculosis4, Escheria coli5, Helicobacter pylory6. In particular, one of the bioactive compounds, artemisinin7, has effective results against Gram-positive bacteria, and its antibacterial activity is similar to streptomycin (bactericide). Wormwood is also recommended due to its immunomodulatory, antiviral, antifungal, antiparasitic, anti-inflammatory, antioxidant effects8.

Turmeric is a panacea, the over 7 thousand studies conducted with this plant, showing countless beneficial effects (Alzheimer’s9, pancreatic/ lung/ breast cancer10, inflammatory diseases – rheumatoid arthritis, osteoarthritis11, skin conditions – Vitiligo12, Psoriasis13). Turmeric is used as a basic ingredient in Asian cuisine, and helps maintain the health of people in areas with poor hygiene.

In Ayurvedic medicine it is used as an antiseptic, antibacterial and anti-inflammatory. Current research suggests positive effects against the genus Salmonella14, and a strong fungicidal action15. Curcumin, the main active ingredient in turmeric, can act not only as an antifungal and antibacterial compound, but also as an antiviral compound, inhibiting replication in a wide range of viruses (Zika16, HSV-117, Influenza A18).

Chemical research has shown the presence of several beneficial compounds, such as flavonoids, ascorbic acid, tocopherols, citric acid, sterols and minerals, in grapefruit seeds. Their efficacy has been reported19 against over 800 bacterial and viral strains, numerous fungi and a large number of unicellular (Entamoeba histolytica20)/ multicellular (Ascaris lumbricoides21) parasites. It has been shown to help inhibit the proliferation of Candida albicans22, a yeast that can affect probiotic bacteria and affect the health of the gastrointestinal tract. Naringenin, a bioactive component of grapefruit seeds, showed gastroprotective activity, especially by inhibiting Helycobacter pylori23.

Black garlic is obtained from fresh garlic (Allium sativum L.) which has been fermented for a period of time at a controlled high temperature (60–90 ° C) and under controlled high humidity (80–90%). Compared to fresh garlic, black garlic is sweeter, does not release a strong flavor due to its low allicin content, but has increased bioactivity, the phenolic content is 5-8 times higher than that of fresh garlic, so it has an activity much higher antioxidant24. In terms of its medicinal properties, black garlic has been described as antimicrobial, antiseptic, antiviral, antioxidant, immunostimulant, cardioprotective, hypoglycemic and anticancer25. Its properties are related to the bioactive molecules of garlic, such as its organosulfur compounds and non-volatile amino acids (thiosulfinates). These bioactive molecules also include reducing sugars, minerals, saponins and vitamins (A, B and C complex). Black garlic significantly suppresses the growth of the fungus Penicillium citrium26 and also acts on the bacteria Staphylococcus aureus, Escherichia coli, and Bacillus subtilis27.

Cordyceps, considered the mushroom of kings / gods, is the most famous medicinal mushroom being used since antiquity in Tibet. Over the last few years, a series of studies have been carried out to prove its antibacterial and antiviral properties. The results show the existence of the antibacterial polysaccharide extracted from the Cordyceps fungus, which is able to modify the microbial cell permeability28 (Escherichia coli, Staphyloccocus aureus, Bacillus subtilis, Salmonella paratyphi and Pseudomonas aeruginosa29). In addition to the results of antimicrobial activity, cordimin (antifungal peptide) has been isolated, which inhibits the mycelial growth of Bipolaris maydis, Mycosphaerella arachidicola, Rhizoctonia solani and Candida albicans30.

Ganoderma, known as the „mushroom of immortality”, contains over 40031 bioactive compounds needed to maintain the body’s health, including triterpenoids, polysaccharides, nucleotides, sterols, fatty acids and proteins / peptides, which have a number of medicinal effects, mainly antimicrobial (Staphylococcus aureus, Proteusbacillus vulgaris, Bacillus subtilis32), antifungal (Candida albicans33), antiviral (especially against herpes34 and HIV35), anti-inflammatory, antioxidant, as well as strengthening the immune system36 (activation of macrophages by polysaccharides, induces the release of cytokines that play a role in transmitting information between immune system cells and leukocytes).

Fermentation37 creates the necessary supply of pre / pro / parapro / postbiotics, which ensures both antiviral and antibacterial effects and the normalization of the intestinal microbiota. In traditional fermentation, unlike industrial fermentation, we will find a multitude of bacteriophages with beneficial regulatory effects on the microbiome.

A mixture of fermented plants in SCOBY cultures was also used: Green tea (Camellia sinensis); Neem (Azadirachta Indica); olive leaves (Olea europaea); Bitter cucumber (Momordica Charantia); Mint (Mentha piperita) – cold plant, fights the inflammation that accompanies the infection; Black cumin (Bunium persicum) – strong antioxidant38 and antifungal39 especially inhibitory effect on Fusarium oxysporum; Oregano (Origanum vulgare) – hepatoprotective element40, antifungal (Aspergillus niger)41, antibacterial (Salmonella enteritidis)42 and strong antioxidant43; Licorice (Glycyrrhiza glabra).

These are an excellent source of vitamins (A, B1, B6, B9, B12, C, E, K), minerals (calcium, magnesium, potassium, sodium, iron and zinc-antiviral element).

Also, by the presence in the composition of: Propolis which has a strong antiviral effect44 (Varicella zoster (VZV), adenovirus type 5 (ADV), HSV1 (Herpex simplex virus)), antimicrobial effect45 (Enterococcus faecalis), antifungal46 (Cryptococcus neoformans), immunomodulatory, anti-inflammatory effect; Amrita® „food of the gods” – polyfoam pollen fermented in SCOBY consortium, is rich in polyphenols, flavonoids, organic silicon, vitamins, in addition it has a high content of active substances (antioxidants, hydroxy acids, SCFA- butyric acid favors a normal intestinal flora which in turn determines a high immunity); Shilajit & Triphala maintains and promotes a normal intestinal microbiome, Shilajit „mountain blood” the result of fermentation of plant mass at extremely high pressures, over a long period of time is the main source of humic substances (humic acid, fulvic acid)47 that play a role in stimulating the system immune (health enhancer)48, strong antioxidant49, antiviral (decreases HIV infection and replication, inhibits viral fusion between T44 cells)50 and improves cognitive processes51; The acetic acid resulting from the fermentations ensures the inhibition of the formation of the biofilm necessary for the development of pathogens (Streptococcus pyogenes, Pseudomonas, etc.).

In the composition of Bacteriocin 3xBiotics we find many active principles that have as main action the inhibition of bacterial activity, viral (recent studies at Rockeffeller University have shown that there are 3 bacteria in the intestinal microbiome whose metabolites, isopentenyl adenosine, pyrazine, 5-hydroxytryptamine, have the effect of blocking more than 90% of SARS-CoV-252) and fungal infection.

Bacteriocin 3xBiotics becomes an ideal therapeutic option for patients who have intolerances, allergies to the administration of classic antibiotics or who have developed resistance to them.

Bibliographical references

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