从初乳中获得的多功能产品发酵的开菲尔粒
Eureka项目标识:E! 11785
罗马尼亚融资合同号62/2018
罗马尼亚融资机构:高等教育、研究、发展和创新融资执行单位 (UEFISCDI)
项目价值(罗马尼亚):412 900 欧元(322 578 欧元来自国家预算,90 322 欧元共同融资)
我们的项目
抽象的:
3-Biotic+ 项目旨在开发一种复杂、多功能的 3-Biotic 产品(具有益生元、益生元和后生元作用),以生物活性肽为标准化,通过使用增强型开菲尔细菌群落控制初乳发酵。该产品将在实验开发阶段进一步开发一系列新的营养保健品和药妆品。该项目的目标是:(i)选择增强的开菲尔细菌群落,能够通过发酵产生生物活性肽和其他生物活性化合物进入初乳; (ii) 优化发酵过程并配制用于营养保健品和药妆品应用的所得产品; (iii) 展示由增强型开菲尔细菌聚生体发酵的初乳获得的产品的功能。一种创新方法专注于生物活性肽,作为复杂的三生物产品标准化的关键组成部分。其他创新是复杂多功能产品的整体方法,符合综合和个性化健康治疗的实际趋势。此外,还设想了项目合作伙伴(罗马尼亚的一家中小企业、一所大学和一家研发机构以及挪威的一家中小企业)之间的研究协调和活动关联。项目从TRL3技术水平起步,项目实施阶段(实验开发)将提升至TRL7/TRL8水平。主要成果是一种多功能、三生物产品,采用生物活性肽标准化。这种三生物产品将进一步纳入营养保健品和药妆品的特定收据中,并由项目合作伙伴申请专利。中小企业的市场地位将通过其创新产品范围的多元化得到巩固。
项目合作伙伴
- Medica Laboratories Srl,罗马尼亚, 主要合作伙伴,缩写医学(www.pro-natura.ro)
- “下多瑙河”大学加拉茨,罗马尼亚,合作伙伴1,缩写UDJG(www.ugal.ro)
- NorGenoTech AS,挪威,合作伙伴 4,P4,缩写 NGT(www.norgenotech.no/)
- 罗马尼亚国家生物科学研究与发展研究所,缩写为 INCDSB(www.incdsb.ro)
目标
该项目 3-生物+ 旨在通过使用增强型开菲尔联合体对初乳进行控制发酵,开发一种复杂的多功能三生物产品,该产品采用生物活性肽标准化。该产品将进一步开发一系列营养保健品(食品/膳食补充剂、运动员功能性食品)和药妆品。
该项目的具体目标是::
(i) 选择增强型开菲尔联合体,能够通过发酵将生物活性肽和其他生物活性化合物生成到初乳中;
(ii) 优化发酵工艺并配制用于营养保健品和药妆品应用的所得产品;
(iii) 展示从增强型开菲尔菌群发酵的初乳中获得的产品的功能。
该工作包 实现具体项目目标所必需的是:
WP1。增强型开菲尔联合体的选择
WP2。发酵和配方工艺的开发
WP3。所形成的生物活性肽的靶向表征及其在营养保健品和药妆品中的作用
WP4。与选定的生物应用相关的抗突变、抗原毒性和抗炎作用的测定
WP5。确定生物后效应赋予营养保健品更高的价值
WP6。传播和利用
WP7。管理
The relationship between objectives and work-packages is presented in bellow figure:
“ 3-biotic +”项目目标与工作包之间的关系。
结果
预期成绩
The main result of this project will be a multifunctional, 3-biotic product, standardized in bioactive peptides. This 3-biotic product will be further included into specific receipts of nutraceuticals and cosmeceuticals and patented by the project partners. We expect to obtain:
- 高通量筛选技术
- 很好地表征增强酸牛奶谷物
- 为在发酵之前初乳调理选择前处理工艺
- 初乳比例放大产量通过在最佳条件克菲尔谷物发酵
- Stable, long shelf life 3biotic product
- 基于初乳3-生物产物的标准化方法通过克菲尔谷物发酵
- DNA lesions and oxidative DNA damage results validated, data quality validated.
- Mutagenesis results validated (OECD 476)
- 进度报告
- 提交出版的文章
- 科学通讯
- 创新的技术解决方案保护
获得的结果
The results obtained in the 1st stage of the project are available 这里.
The results obtained in the 2nd stage of the project are available 这里.
The results obtained in the 3nd stage of the project are available 这里.


DISSEMINATION OF THE 2nd STAGE RESULTS
Papers:
- Mihaela Cotârleț, Aida Mihaela Vasile, Alexandra Gaspar-Pintiliescu, Anca Oancea, Gabriela Elena Bahrim, Tribiotic kefir production from bovine colostrum using artisanal and selected starter cultures, manuscript submis la CyTA – Journal of Food, cod submitere TCYT-2019-0228
- Alexandra Gaspar-Pintiliescu, Anca Oancea, Mihaela Cotarlet, Aida Mihaela Vasile, Gabriela Elena Bahrim, Sergey Shaposhnikov, Oana Craciunescu, Elena Iulia Oprita, Angiotensin-converting enzyme inhibition, antioxidant activity and cytotoxicity of bioactive peptides from fermented bovine colostrum, 2019, International Journal of Dairy Technology, Early Access: OCT 2019, DOI: 10.1111/1471-0307.12659
- Mihaela Cotârleț, Aida Mihaela Vasile, Alina Mihaela Cantaragiu, Alexandra Gaspar-Pintiliescu, Oana Craciunescu, Anca Oancea, Angela Moraru, Ionuț Moraru, Gabriela Elena Bahrim, Colostrum-derived bioactive peptides obtained by fermentation with kefir grains enriched with selected yeasts, The Annals of the University Dunarea de Jos of Galati, Fascicle VI – Food Technology, 2019, 43 (1), 54-68, DOI: 10.35219/foodtechnology.2019.1.04
Communications:
- Gabriela Elena Bahrim, Aida Mihaela Vasile, Mihaela Cotârleţ, Cultură starter artizanală, produs liofilizat din granulele de chefir de lapte, 2019, UGAL INVENT-Salonul inovării şi cercetării, 16-18 Octombrie, Galaţi
- Gabriela Bahrim, Georgiana Horincar, Mihaela Cotârleț, Aida Vasile, Oana Craciunescu, Anca Oancea, Nicoleta Stănciuc, Gabriela Râpeanu, Iuliana Aprodu, 2019, Yarrowia lipolytica as valuable workhorse for postbiotics production, 2nd ICGEB Workshop “Modern Biotechnological Advances For Human Health – BAHH”, 28-31 Mai, București. România
- Gabriela Elena Bahrim, Mihaela Cotârleț, Alexandra Gaspar-Pintiliescu, Oana Craciunescu, Anca Oancea, Aida Mihaela Vasile, 2019, Tribiotics design from bovine colostrum as a couple of multiple metabolic activities of the artisanal and selected cultures, 10th Probiotics, Prebiotics &New Foods, Nutraceuticals And Botanicals For Nutrition & Human And Microbiota Health Congress, 8-10 Septembrie, Roma, Italia
- Utoiu E, Gaspar A, Craciunescu O, Sanda C, Oprita EI, Oancea A, Moraru A, Cotarlet M, Mustatea G, Shaposhnikov S, Bioactive peptides from colostrum fermented with enhanced kefir consortia: biochemical characterization and specific biological activity, Agriculture for Life, Life for Agriculture 2019, AgroLife 2019, 6-8 June 2019, Bucharest, Romania
- Anca Oancea, Ionut Moraru, Gabriela Bahrim, Oana Craciunescu, Alexandra Gaspar-Pintiliescu, Viorica Coroiu, Mihaela Enache, Angela Moraru, Florin Oancea, A new nutraceutical product prepared by colostrum fermentation biotechnology, 2nd ICGEB Workshop “Modern Biotechnological Advances for Human Health – BAHH”, 28 – 31 May 2019, Bucharest, Romania
- Anca Oancea, Elena Utoiu, Teodora Ciucan, Oana Craciunescu, Alexandra Gaspar-Pintiliescu, Viorica Coroiu, Ana Maria Stanciuc, Laura Mihaela Stefan, Comparative studies concerning the bioactivity of peptides obtained by kefir-kombucha fermentation of bovine colostrum, 10 th PROBIOTICS, PREBIOTICS &NEW FOODS, NUTRACEUTICALS AND BOTANICALS FOR NUTRITION & HUMAN AND MICROBIOTA HEALTH CONGRESS, 8-10 Septembrie 2019, Roma, Italia
Patent demands:
- Gabriela Elena Bahrim, Aida Mihaela Vasile, Mihaela Cotârleţ, Artisanal starter culture, lyophilized product from kefir grains) – patent demand A / 20470/05.08.2019, gold medal at INFOINVENT, XVI nd ediition, 20-23 november 2019, Chişinău, Republic of Moldova.
- Angela Moraru, Ionut Moraru, Gabriela Elena Bahrim, Aida Mihaela Vasile, Mihaela Cotârleţ, Anca Oancea, Process for fermentation of colostrum with enhanced kefir granules, Patent demand no. A/00826/28.11.2019

SEM microscopy images showing lactobacilli adhered to the intestinal epithelial cells membrane in the presence of peptides from fermented colostrum.

Appearance of freeze-dried kefir starter culture

SEM image of fermented product obtained by kefir fermentation with artisanal lyophilized starter culture.

Colostrum fermentation technology



DISSEMINATION OF THE 3nd STAGE RESULTS
Papers
Mihaela Cotârleţ, Aida Mihaela Vasile, Alexandra Gaspar-Pintiliescu, Anca Oancea, Gabriela Elena Bahrim, Tribiotication strategy for the functionalization of bovine colostrum through the biochemical activities of artisanal and selected starter cultures, 2020, CyTA – Journal of Food, 18:1, 274-280, Received 28 Oct 2019, Accepted 13 Mar 2020, Published online: 27 Apr 2020
Mihaela-Aida Vasile, Mihaela Cotârleţ, Gabriela-Elena Bahrim, Metabolic functionality of the synergistically ameliorated consortium based on wild kefir grains and selected microorganisms for bovine colostrum fermentation, in press in Innovative Romanian Food Biotechnology, Vol. 19, 2020
Alexandra Gaspar-Pintilescu, Angela Moraru, Anca Oancea, Iulia Oprița, Elena Uțoiu, Teodora Ciucan, Laura Mihaela Stefan, Ana Maria Stanciuc, Ana-Maria Seciu-Grama, Gabriela Bahrim, Florin Oancea, Functional food produced from colostrum by fermentation with enhanced micro-organisms consortia, send for publication at Foods, MDPI, 2020 IF= 4,092 (under evaluation)
Communications
Ionuț MORARU, Angela MORARU, Anca OANCEA, Alexandra GASPAR-PINTILIESCU, Viorica COROIU, Laura Mihaela STEFAN, Andreea IOSĂGEANU, Teodora CIUCAN, Daniela ILIE, Cătălina SANDA, Ana-Maria SECIU-GRAMA, Nutraceutical and cosmeceutical products containing low molecular weight peptides from fermented colostrum, “Priorities of Chemistry for a Sustainable Development” – PRIOCHEM XVI, 28-30 October, Bucharest, 2020
Patent demand
Anca Olguta Oancea, Lucia Moldovan, Oana Craciunescu, Laura Mihaela Stefan, Andreea Iosageanu, Ionut Moraru, Gabriela Elena Bahrim, Cosmeceutic composition with moisturizing, protective and emollient effect, OSIM.

Dr. Ionut Moraru
罗马尼亚SC Medica Laboratories SRL项目经理
ionutmoraru@pro-natura.ro〜 www.pro-natura.ro
ionutmoraru@pro-natura.ro〜 www.pro-natura.ro
Prof. dr. Gabriela-Elena Bahrim
Project responsible “Dunărea de Jos” University Galați, Romania
gabriela.bahrim@ugal.ro〜 www.ugal.ro
Dr. Sergey Shaposhnikov
Project responsible NorGenoTech AS, Norway
sas@norgenotech.com〜 www.norgenotech.no
Dr. Anca Oancea
Project responsible National Institute for Research and Development for Biological Sciences, Romania
oancea.anca@gmail.com〜 www.incdsb.ro